[HELICONIUS] W-linked markers -- sexing young stages of Heliconius

[Durrell Kapan]

Hi all,

Larry shared this reference with me a while back. Here are the scans all ready to post on the site.

As you can see, in the females there is a small line that crosses between the last two complete segments. 

Durrell
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On Apr 14, 2010, at 5:23 AM, James Mallet wrote:

> It's easy to tell apart the sexes of pupae of most Leps with a bit of practice via morphology and a hand lens, so you don't need genetics or to sample destructively.  
> 
> For Heliconius pupae, there's a nice diagram showing sexual differences of ?Heliconius erato in one of Jocelyn Crane's papers -- would anyone like to put it on the web? I haven't got Crane's papers here, but I think it's probably in this one:
> 
> Beebe, W., J. Crane, and H. Fleming. 1960. A comparison of eggs, larvae and pupae in fourteen species of Heliconiine butterflies from Trinidad, W.I. Zoologica, New York 45:111-153.
> 
> I understand it's also possible to tell apart larvae of some Lepidoptera, but I don't think anyone has checked carefully in Heliconius. In small transparent-cuticled leps like tortricids it's possible to see the dark coloured testes  through the cuticle in quite young larvae. Igor Emelianov and I used this in Zeiraphera diniana. (Testes are also dark in Heliconius adults -- not yellow or white as suggested by Alexie for I presume other taxa).
> 
> Ilik Saccheri claims (pers. comm.) that you can take small amounts of haemolymph from Biston pupae without killing them.
> 
> Good luck, Jim
> 
> 
>> On Wed, 2010-04-14 at 11:57 +0100, Jamie Walters wrote: 
>>> Hello all,
>>> 
>>> I'm curious to know if anyone has had any success (or informative  
>>> failures...?) with developing W-linked markers for Heliconius (or
>>> any  
>>> butterfly that would be near enough to be useful).
>>> 
>>> Or, alternatively, if anyone has any particularly clever ideas for  
>>> mining the existing genome data for candidate regions I'd be keen
>>> to  
>>> hear them.
>>> 
>>> I'm simply interested in being able to sex juveniles and pupae.
>>> 
>>> 
>>> Thanks very much,
>>> 
>>> Jamie
>>> 
>>> _______________________________________________
>>> HELICONIUS mailing list
>>> 
>>> HELICONIUS at ucl.ac.uk
>>> 
>>> http://www.mailinglists.ucl.ac.uk/mailman/listinfo/heliconius
>>> 
>> 
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> ________________________________
> 
> James Mallet
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> 
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