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There was a previous correspondence about sexing pupae. See
<a class="moz-txt-link-freetext" href="http://www.mailinglists.ucl.ac.uk/pipermail/heliconius/2010-April/000077.html">http://www.mailinglists.ucl.ac.uk/pipermail/heliconius/2010-April/000077.html</a><br>
<br>
The testes in Heliconius if I remember rightly are fused into a
single blob and bright red, and they're usually visible in the
abdomen of dissected lepidopterous larvae as well as pupae (though
I've never checked in Heliconius).<br>
<br>
However, you should be able to sex the pupae externally with 100%
accuracy with a handlens! It just takes some practice with your
particular species. Much better if you don't want to mangle the
specimen!<br>
<br>
See:
<a class="moz-txt-link-freetext" href="http://www.ucl.ac.uk/taxome/jim/Mim2/heliconius_pupa_sex_difference.html">http://www.ucl.ac.uk/taxome/jim/Mim2/heliconius_pupa_sex_difference.html</a><br>
<br>
jim<br>
<br>
<div class="moz-cite-prefix">On 10/05/2013 09:18, Richard Merrill
wrote:<br>
</div>
<blockquote
cite="mid:FAC2BA31-21E2-44A1-A84E-160A1031FEA5@zoo.cam.ac.uk"
type="cite">
<meta http-equiv="Content-Type" content="text/html;
charset=ISO-8859-1">
Hi All,
<div><br>
</div>
<div>I was wondering whether anyone had any experience of sexing
pupae by dissecting them (and if so is it possible to do this
for early and late stage pupae). </div>
<div><br>
</div>
<div>I know it is meant to be possible from external morphology,
and I've done this before but only with ~50% accuracy!</div>
<div><br>
</div>
<div>Best wishes,</div>
<div><br>
</div>
<div>Richard</div>
<div><br>
</div>
<div><br>
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<span
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11px; ">Tel:
(+44)(0)1223
336644</span><br
style="font-size:
11px; ">
<span
class="Apple-style-span"
style="font-size:
11px; ">Mob: (+44)(0)7590
984754</span></div>
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class="Apple-style-span" style="font-size: 11px; ">Email: <a
moz-do-not-send="true"
href="mailto:r.merrill@zoo.cam.ac.uk">r.merrill@zoo.cam.ac.uk</a></span><br
style="font-size:
11px; ">
<span
class="Apple-style-span"
style="font-size:
11px; ">Web: <a
moz-do-not-send="true"
href="http://heliconius.zoo.cam.ac.uk/2009/richard-merrill/">http://heliconius.zoo.cam.ac.uk/2009/richard-merrill/</a></span><br
style="font-size:
11px; ">
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<pre wrap="">_______________________________________________
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<pre class="moz-signature" cols="72">--
James Mallet
OEB Dept, Harvard University
& Professor of Biological Diversity,
University College London
16 Divinity Avenue
Cambridge, MA 02138, USA
<a class="moz-txt-link-abbreviated" href="http://www.ucl.ac.uk/taxome/jim">www.ucl.ac.uk/taxome/jim</a></pre>
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